| 2 EBP has developed over time to now integrate the best research evidence, clinical expertise, the patient's individual values and circumstances, and the characteristics of the practice in which the health professional works | For example, some antibodies are ineffective in Western blotting WB but are suitable for immunohistochemistry IHC applications, because, in the IHC procedure, a complex antigenic site might be maintained in the tissue, whereas in the WB procedure, the process of sample preparation alters the protein conformation sufficiently to destroy the antigenic site, and hence eliminates antibody binding |
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| The epitope is then inaccessible to the antibody in a nondenaturing protocol, such as immunoprecipitation | Thus, given enough time, just about any foreign substance will be identified by the immune system and evoke specific antibody production |
| evidence-based medicine: principles for applying the users' guides to patient care | Because antigen molecules exist in space, the epitope recognized by an antibody may be dependent upon the presence of a specific three dimensional antigenic conformation e |
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| Epitopes The small site on an antigen to which a complementary antibody may specifically bind is called an epitope or antigenic determinant | Patients expect to receive the most effective care based on the best available evidence |
1 The tutorial focuses largely on efficient literature searching and therefore on asking questions and acquiring the best evidence.
20| Primary culture refers to the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate i | Normal cells usually divide only a limited number of times before losing their ability to proliferate, which is a genetically determined event known as senescence; these cell lines are known as finite |
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| Is there evidence that can guide me to do this in a more effective way? , passaged by transferring them to a new vessel with fresh growth medium to provide more room for continued growth | Optimally, an antibody that recognizes a linear epitope on the surface of a normally folded protein will work well in both nondenaturing and denaturing protocols |
Contact hours and workload This module is approximately 150 hours of work.
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